I noticed that during that first load (after multiple tests), that the userGameState.cgs file from the Cities_Skylines folder is missing. Meanwhile, all of my assets have been automatically enabled (which was not the case before the update). When the game opens at first, the area in the content manager for mods is completely blank none of my Workshop subscribed mods appear and even the built-in features like infinite money and hard mode are gone. Issue started as soon as my game updated to the most recent version (and I had already done my homework by replacing all the mods that were listed incompatible early with the new compatible versions as well as unsubscribing mods that were suspected to be broken but no replacement yet).